UJI EFEK KOMBINASI EKSTRAK DAUN SELEDRI (Apium graveolens L.) DAN EKSTRAK DAUN KEMANGI (Ocimum bacilicum L.) TERHADAP KADAR ASAM URAT DARAH PADA MENCIT (Mus musculus L.) YANG DIINDUKSI KALIUM OKSONAT

Abstract

Latar Belakang : Asam urat merupakan penyakit yang menyerang persendiaan tubuh, seperti jari tangan, tumit, jari kaki, lutut, hingga pergelangan tangan. Penyakit timbul karena adanya penumpukan zat purin yang kemudian berubah menjadi asam urat. Tumbuhan seledri dan kemangi berpotensi sebagai obat tradisional dalam menurunkan kadar asam urat. Karena tumbuhan tersebut mengandung senyawa flavonoid yang berpotensi sebagai antihiperurisemia dalam menurunkan kadar asam urat.

Tujuan Penelitian : Untuk mengetahui efek pemberian kombinasi ekstrak etanol daun seledri dengan daun kemangi dan dosis efektif terhadap penurunan kadar asam urat.

Metode Penelitian : Penelitian ini bersifat eksperimental dengan teknik pengambilan sampel secara purposive sampling yang terdiri dari kelompok perlakuan kontrol negatif (Na-CMC 5%), kontrol positif (allopurinol), kelompok 1 dosis 75 : 25 mg/kg BB, kelompok 2 dosis 50 :50 mg/kg BB dan kelompok 3 dosis 25 : 75 mg/kg BB. Penelitian ini dilakukan pada bulan Juli hingga Agustus 2023. Hasil Penelitian : Hasil penelitian menunjukkan bahwa kombinasi ekstrak etanol daun seledri dan daun kemangi mampu menurunkan kadar asam urat pada mencit. Perlakuan kombinasi ekstrak etanol daun seledri dan daun kemangi menunjukkan penurunan kadar asam urat sejak H-9 hingga H-15. Kombinasi ekstrak etanol daun seledri dan daun kemangi berpengaruh secara signifikan terhadap penurunan kadar asam urat mencit karena nilai sig > 0,05. Kesimpulan : Kombinasi ekstrak etanol daun seledri dan daun kemangi efektif dalam menurunkan kadar asam urat sekitar 21 % hingga 27% dan dosis yang paling efektif yaitu kombinasi dosis 50 :50 mg/kg BB. 

Kata Kunci : Asam Urat, Antihiperurisemia, Allopurinol, Kemangi, Seledri

Background : Gout is a disease that attacks the joints of the body, such as fingers, heels, toes, knees, to the wrists. The disease arises due to a buildup of purine substances which then turn into uric acid. Celery and basil plants have potential as traditional medicines for lowering uric acid levels. Because this plant contains flavonoid compounds which have the potential to act as antihyperuricemia in reducing uric acid levels. Research Objectives : To determine the effect of giving a combination of ethanol extract of celery leaves with basil leaves and the effective dose on reducing uric acid levels.Research Methods : This study was an experimental study with a purposive sampling technique consisting of a negative control group (Na-CMC 5%), positive control (allopurinol), group 1 dose 75: 25 mg/kg BW, group 2 dose 50 : 50 mg/kg BW and group 3 dose 25 : 75 mg/kg BW. This research was conducted from July to August 2023. Results : The results showed that the combination of ethanol extracts of celery leaves and basil leaves was able to reduce uric acid levels in mice. The combination treatment of ethanol extract of celery leaves and basil leaves showed a decrease in uric acid levels from D-9 to D-15. The combination of ethanol extract of celery leaves and basil leaves had a significant effect on The combination of ethanol extract of celery leaves and basil leaves had a significant effect on reducing uric acid levels in mice because the sig value was > 0.05. Conclusion : The combination of ethanol extract of celery leaves and basil leaves is effective in reducing uric acid levels around 21% to 27% and the most effective dose is the combined dose of 50:50 mg/kg BW.

Keywords    : Uric Acid, Antihyperuricemia, Allopurinol, Basil, Celery