Isolasi Senyawa Fenolik dari Buah Punti (Diploknema oligomera H.J.Lam) dengan Analisis FT-IR
Abstract
Penelitian tentang buah punti (Diploknema oligomera H.J. Lam) telah dilakukan. Hasil skrining fitokimia fenolik ekstrak etanol dengan penambahan FeCl3 5% memberikan warna hitam kehijauan menunjukkan bahwa ekstrak etanol Diploknema oligomera positif mengandung fenolik. Dari 44,13 gram ekstrak etil asetat isolat diploknema oligomera dihasilkan senyawa berupa pasta berwarna kekuningan sebanyak 0,0025 gram dengan eluen n-Heksana: Etil Asetat 80:20 (v v). Hasil analisis dengan Infrared Spectrocopy (FT-IR) menunjukkan hasil isolasi Diploknema oligomera merupakan senyawa fenolik, dengan spektrum yang identik menunjukkan adanya kandungan fenol yang diperkuat dengan adanya gugus OH pada pita serapan luas yang ditunjukkan pada bilangan gelombang 3252,12 cm-1 dan 3468,16 cm-1, C-H aromatik pada 723,34 cm-1, C = O karbonil pada 1734, 08 cm-1, dan pada pita serapan 1263,43 cm-1yang menunjukkan adanya gugus C-O.
Kata kunci: Diploknema oligomera, isolasi, skrinning, spektroskopi infra merah (FT- IR)
Research on punti fruit (Diploknema oligomera H.J. Lam) has been done. Phytochemical Screening results of phenolic ethanol extract with addition of FeCl3 5% gave the greenish black color indicated that ethanol extract Diploknema oligomera was positive to phenolic. The resulting isolate of 44,13 g of Diploknema oligomerais a yellowish, pictorial paste, yielding as much as 0,0025 gram with n- Hexane:Ethyl Acetate eluent 80:20 (v/v). Results of analysis with Infrared Spectrocopy (FT-IR) showed the results of the Diploknema oligomera which is phenolic compounds, with identic spectrum shows the presence of phenol content which is strengthened by the presence of an OH group in the broad absorption band shown in wave numbers 3252.12 cm-1 and 3468.16 cm-1, C-H aromatic at 723.34 cm- 1, C = O carbonyl at 1734 , 08 cm-1, and in the absorption band1263.43 cm-1 indicated the presence of C-O groups.
Keywords: Diploknema oligomera, isolation, screening, Infrared Spectrocopy (FT- IR)
Full Text:
PDFReferences
Adhikari MK, Shakya DM, Kayastha M, Baral SR, Subedi. MN. (2007). Bullentin of Department of Plant Resources No. 28. Medicinal Plant of Nepal (revised). Department of Plant Resources, Kathmandu, p. 112.
Anam, Choirul. Sirojudin, K, Sofjan Firdausi. (2007). Analisis Gugus Fungsi Pada Sampel Uji, Bensin Dan Spiritus Menggunakan Metode Spektroskopi FT-IR. Berkala Fisika. Vol 10 no.1. Hal. 79 – 85.
Awasthi YC, Mitra CR. (1968). Madhuca butyracea. Costituents of the fruit pulp and the bark. Phytochemistry 7. Hal.637-640.
Awasthi YC, Mitra CR. (1962). Flavonoidsof Madhuca butyracea nut shell. J Org Chem 27. Hal. 2636-2637.
Banerji R, Mishra G, Nigam SK. (1985). Butyracic acid, a new sapogenin from Madhuca butyraceae. Planta Med 51. Hal.280-281.
Chanwitheesuk A, Aphiwat Teerawutgulrag, Jeremy D Kilburn, Nuansri Rakariyatham, 2005. Antimicrobial gallic acid from Caesalpinia mimosoides Lamk.Food Chemistry
1044–1048.
Chusnul, (2011). Spektroskopi IR. www. Scribd.com diakses tanggal 08 Juli 2020. Devkota, et al., (2012). Studies on Medicinal Plant Resources of the Himalayas: GC-
MC Analysis of Seed Fat of Chyuri (Diploknema butyracea) from Nepal. Pharmacognocy Journal. Vol.4. Issue 27. Hal. 42
Febriyantie, Vania. (2014). STUDI KEKERABATAN FENETIK BEBERAPA
JENIS TANAMAN SAWO Pouteria (Sapotaceae) MENGGUNAKAN METODE RAPD. Universitas Pendidikan Indonesia | repository.upi.edu | perpustakaan.upi.edu mm
H. Schulz, M. Baranska, Vib. Spectrosc., 43 (2007) 13-25.
J. Wiley & Sons, Chichester, 1997.
Khetwal KS, Verma DL. (1986). Flavonoids from the flowers of Diploknema butyracea. Fitoterapia57. Hal.128
Kristanti Alfinda N, et al., (2008). Buku Ajar Fitokimia. Airlangga University Press.
Surabaya.
Leopoldini, M., Russo, N., & Toscano, M. (2011). The molecular basis of working mechanism of natural polyphenolic antioxidants. Food chemistry,125(2), Hal. 288-306.
Li X, Liu Y, Wang D, Yang C, Nigam SK, Mishra G. (1994). Terpenoid saponins from madhuca butyracea. Phytocemistry 37. Hal. 827-829.
Manach, C, Augustin Scalbert, Cristine Morand, Cristian Remesy. (2004). Polyphenol: food sources and bioavaibility. American journal of Clinical Nutrition. 79 (5), 727-747.
Manandhar NP. Plants and People Of Nepal. (2002). Timber Press, Inc p. 205. Mishra G, Benerji R, Nigam SK. (1991). Butyraceol, a triterpenoidal sapogenin from
Madhuca butyracea. Phytochemistry 30: Hal.2087-2088.
Nigam SK, Li X, Wang D, Mishra G, Yang C. (1993). Triterpenoidal saponins from Madhuca Butyracea. Phytochemistry 31 Hal. 3169-3172.
Ouissam Abbas, G´eraldine Comp`ere, Yvan Larondelle, Darly Pompeu, Herv´e Rogez, Vincent Baeten, (2017). Phenolic compound explorer: a mid-infrared spectroscopydatabase,Vibrational Spectroscopyhttp://dx.doi.org/10.1016/j.vibspec.2017.05.008
R. Świsłocka, E. Regulska, M. Samsonowicz, W. Lewandowski, J. Mol. Struct., 1044 (2013) 181-187.
Zhu, F. (2015). Intractions between starch and phenolic coumpound. Trends in Food Science &Te`Xchnology, 43 (2), Hal. 129-143.
DOI: https://doi.org/10.33143/jhtm.v10i1.4193
Refbacks
- There are currently no refbacks.
e-ISSN: 2615-109X
p-ISSN: 2442-4706
Principal Contact
Direktorat Penelitian dan Pengabdian Masyarakat (DPPM)
Universitas Ubudiyah Indonesia - Jl. Alue Naga Desa Tibang, Banda Aceh 23114, Indonesia
Phone: 0651-7555566
Fax: 0651-7555566
Email: dppm@uui.ac.id
This work is licensed under a Creative Commons Attribution 4.0 International License